Assistant Professor Dr. Kornsorn Srikulnath

  • Position:
    • Vice Head of Department of Genetics (Research section)
    • Assistant Professor (Kasetsart University)
    • Researcher (Reptile Cytogenetics, Nagoya University, Japan) at Prof.Yoichi Matsuda’s lab
    • Associate Editor: Genetics and Genomics (formerly name Thai Journal of Genetics)
    • Associate Editor: Agriculture and Natural Resources
    • Editorial Board: Genes and Genomics (section Phylogenomics, Conservation Genetics, Diversity)
    • Board Committee of Genetics Society of Thailand
    • International Steering Committee of Asian Chromosome Colloquium
  • Office number: MG 4516
  • Lab number: MG4611 (Laboratory of Animal Cytogenetics & Comparative Genomics)
  • E-mail: kornsorn.s@ku.ac.th; ksrikulnath@yahoo.com
  • Contacts: +66-25625444 ext.4240
  • Education:
  • 2012 Postdoctoral fellow (Reptile Cytogenetics), Nagoya University, Japan
    2010 Ph.D. (Genetics), Kasetsart University, Thailand
    2005 B.SC. (Biology), 1st honor, Kasetsart University

     

    Research interestsGrantsAdditional informationPublication

    The aim of my study is to clarify genome and chromosome structures as well as their evolutionary processes in vertebrates by cytogenetic and molecular biology techniques. I plan to carry out the following research topics:-

    1. Karyological characterization in vertebrates

      To reveal the karyological characterization in vertebrates, the karyotyping, chromosome banding and FISH mapping are performed. The karyological characterization data would inform us the phylogenetic hierarchy of genome evolution in vertebrates and efficiently sustain the favorable selection in animal breeding program.

    2. Karyotypic and genomic evolution in vertebrates

      To elucidate the process of karyotypic evolution in vertebrates, the chromosome homologies between different species in fish, amphibians, reptiles, birds and mammals are deduced using comparative chromosome mapping.

    3. Origin and differentiation of sex chromosomes, diversity of sex-determining systems and sex-determining gene evolution in vertebrates

      Mammals and birds have a male heterogametic XX/XY-type sex chromosome, and a female heterogametic ZZ/ZW-type sex chromosome, respectively, whereas amphibians have both XX/XY- and ZZ/ZW-type sex chromosome. By contrast, XX/XY- and ZZ/ZW-type sex chromosome not only co-exist in reptiles and fish as genetic sex determination, but the environmental sex determination such as temperature is also found in both vertebrate groups. To clarify the origin and differentiation of sex chromosomes, the comparative chromosome maps of sex chromosomes are constructed and compared them with other species. Furthermore, sex-determining genes such as DM and SOX family are proposed to be a candidate gene of sex determination in vertebrates. The orthologues and paralogues of sex-determining gene, therefore, are studied to disclose gene evolution in vertebrate.

    4. Organization of repetitive element in vertebrate genome

      Repetitive DNA sequences is a good chromosome marker for investigating the process of karyotypic evolution and sex chromosome identification, and for comparing the genomics structure of vertebrate species. This can be also a source for homologous recombination to initiate various categories of chromosomal rearrangements. Here, the characterization and comparison of organized repetitive element among different species should be conducted to find the common and specific repeats in the evolutionary line.

    5. Mitochondrial genome analysis & DNA barcoding

      To clarify the step of evolution in vertebrates, complete mitochondrial genome analysis is used. The structure and organization are compared among different species within the same class. The complete mitochondrial sequence data sets are also scrutinized through cladistic analysis to demonstrate the genetic relationships among them. Furthermore, the sequence of mitochondrial genome is capable to develop molecular barcoding for identifying species.

    Grants

    2013 NRCT fund (National Research Council of Thailand)
    Project: Karyotype of varanid lizards inferred from molecular cytogenetic
    approach
    2013 NRCT fund (National Research Council of Thailand)
    Project: Mitochondrial genome of varanid lizards for evaluation of the
    phylogenetic relationship
    2013 Thailand Research Fund (TRF)
    Project: Thai garlic type classification using electronic nose system
    2013 KURDI fund (Kasetsart University Research and Development Institute)
    Project: Evolution of Fragrance Gene in Aromatic Coconut (Cocos nucifera Linn.)
    2012KURDI fund (Kasetsart University Research and Development Institute)
    Project: Karyological characterization of white sea bass (Lates calcarifer) by molecular cytogenetic approach
    2012 KURDI fund (Kasetsart University Research and Development Institute)
    Project: DNA barcoding and DNA marker of venomous snake in Thailand
    2012Postdoctoral fellowship Nagoya University (Japan)
    Project: Molecular cytogenetics and comparative mapping of amphibians and reptiles
    2012 International collaboration work between Thailand (Kasetsart University) and Japan (Nagoya University)
    Project: A study on genetics and molecular mechanism of hybrid sterility and hybrid dysgenesis in duck

    Awards

    2014 Visiting staff under Lotus Unlimited Project, EU-Asian Mobility (Avian Comparative Genomics), University of Kent, UK approach
    2014 KU Research Star 2013 (Biological Science)

      Courses taught

    • Introduction to Cytogenetics
    • Cytogenetics
    • Principle of Genetics
    • Laboratory in Genetics
    • Intensive Genetics
    • Research Technique in Genetics
      • Students

        1. PhD students

      • Pradipunt Thongthaem Na Ayudhya
      • Sudarat Baichoren
      • Ornjira Prakhongcheep
      • Watcharaporn Thapana
        • 2. Master students

        • Arrjaree Supikamolseni
        • Nampetch Chaiprasertsri
        • Nararat Laopichienpong
        • Parinya Wongtienchai
        • Aorarat Suntronpong
        • Sorravis Lapbenjakul
        • Worapong Singchat
          • 3. Bachelor students

          • Tarada Tripetchr
          • Ponsuda Moonin
          • Utadcha Lerdpisitpaisan
            • 4. Previous students

            • Visarut Chailertrit
            • Nuvadee Bonjongsat
            • Napat Ngaoburanawit
            • Orada Sriwatananukulkit
            • Gunprapa Deein
            • Panupon Twilprawat
            • International publications

              1. Sujiwattanarat, P., P. Pongsanarakul, Y. Temsiripong, T. Temsiripong, C. Thawornkuno, Y. Uno, S. Unajak, Y. Matsuda, K. Choowongkomon and K. Srikulnath. 2016. Molecular cloning and characterization of Siamese crocodile (Crocodylus siamensis) Cu,Zn superoxide dismutase (CSI-Cu,Zn-SOD) gene. Comp. Biochem. Physiol., Part A: Mol. Integr. Physiol. 191: 187-195. DOI: 10.1016/j.cbpa.2015.10.028
              2. Srikulnath, K., W. Thapana, and N. Muangmai. 2015. Role of chromosome changes in crocodilian evolution and diversity. Genomics Inform. (in press).
              3. Unajak, S., N. Pholmanee, N. Songtawee, K. Srikulnath, P. Srisapoome, A. Kiataramkul, H. Kondo, I. Hirono and N. Areechon. 2015. Molecular characterization of Galectin-8 from Nile tilapia (Oreochromis niloticus Linn.) and its response to bacterial infection. Mol. Immunol. 68: 585-596.
              4. Matsubara, K., Y. Uno, K. Srikulnath, Y. Matsuda, E. Miller, M. Olsson. 2015. No interstitial telomeres on autosomes but remarkable amplification of telomeric repeats on the W sex chromosome in the sand lizard (Lacerta agilis). J. Hered. 106: 753-757. DOI: 10.1093/jhered/esv083
              5. Matsubara, K., Y. Uno, K. Srikulnath, R. Seki, C. Nishida, Y. Matsuda. 2015. Molecular cloning and characterization of satellite DNA sequences from constitutive heterochromatin of the habu snake (Protobothrops flavoviridis, Viperidae) and the Burmese python (Python bivittatus, Pythonidae). Chromosoma 124: 529-539. DOI: 10.1007/s00412-015-0529-6
              6. Supikamolseni, A., N. Ngaoburanawit, M. Sumontha, L. Chanhome, S. Suntrarachun, S. Peyachoknagul, K. Srikulnath. 2015. Molecular barcoding of venomous snakes and species-specific multiplex PCR assay to identify seven snake groups for which antivenom is available in Thailand. Genet. Mol. Res. 14: 13981-13997.
              7. Srikulnath, K., Y. Uno, C. Nishida, H. Ota, Y. Matsuda. 2015. Karyotype reorganization in the Hokou Gecko (Gekko hokouensis, Gekkonidae): the process of microchromosome disappearance in Gekkota. Plos ONE 10: e0134829. DOI:10.1371/journal.pone.0134829
              8. Srikulnath, K., S. Sawasdichai, T. K.Jantapanon, P. Pongtongkam, and S. Peyachoknagul. 2015. Phylogenetic relationship of dendrobium species in Thailand inferred from chloroplast matK Gene and Nuclear rDNA ITS Region. Hort. J. 84: 243-252. DOI: 10.2503/hortj.MI-028
              9. Sujiwattanarat, P., W. Thapana, K. Srikulnath, Y. Hirai, H. Hirai, and A. Koga. 2015. Higher-order repeat structure in alpha satellite DNA occurs in New World monkeys and is not confined to hominoids. Sci. Rep. 5: 10315 DOI: 10.1038/srep10315
              10. Trirongjitmoah, S., Z. Juengmunkong, K. Srikulnath, and P. Somboon. 2015. Classification of garlic cultivars using an electronic nose. Comput. Electron. Agric. 113: 148-153. DOI 10.1016/j.compag.2015.02.007
              11. Wannapinpong, S., K. Srikulnath, A. Thongpan, K. Choowongkomon, and S. Peyachoknagul. 2015. Molecular cloning and characterization of the CHS gene family in turmeric (Curcuma longa Linn.). J. Plant Biochem. Biotechnol. 24 (1): 25-33. DOI 10.1007/s13562-013-0232-8
              12. Srikulnath, K., K. Matsubara, Y. Uno, C. Nishida, M. Olsson and Y. Matsuda. 2014. Identification of the linkage group of the Z sex chromosomes of the sand lizard (Lacerta agilis, Lacertidae) and elucidation of karyotype evolution in lacertid lizards. Chromosoma 123: 563-575. DOI 10.1007/s00412-014-0467-8.
              13.Thapana, W., P. Sujiwattanarat, K. Srikulnath, H. Hirai and A. Koga. 2014. Reduction in the structural instability of cloned eukaryotic tandem-repeat DNA by low-temperature culturing of host bacteria. Genet. Res. 96: e13.
              14. Baicharoen, S., T. Miyabe-Nishiwaki, V. Arsaithamkul, Y. Hirai, K. Duangsa-ard, B. Siriaroonrat, H. Domae, K. Srikulnath, A. Koga and H Hirai. 2014. Locational diversity of alpha satellite DNA and intergeneric hybridization aspects in the Nomascus and Hylobates Genera of small apes. Plos One 9: e109151.
              15. Chailertrit, V., A. Swatdipong, S. Peyachoknagul, J. Salaenoi and K. Srikulnath. 2013. Isolation and characterization of ten novel microsatellite markers from Siamese fighting fish (Betta splendens, Osphronemidae, Anabantoidei) and their transferability to related species, B. smaragdina and B. imbellis. Genet. Mol. Res. 13: 7157-7162.
              16. Peyachoknagul, S., C. Nettuwakul, P. Phuekvilai, S. Wannapinpong and K. Srikulnath. 2014. Development of microsatellite markers of vandaceous orchids for species and variety identification. Genet. Mol. Res. 13: 5441-5445.
              17. Peyachoknagul, S., C. Mongkolsiriwatana, S. Wannapinpong, P Srifah Huehne and K. Srikulnath. 2014. Identification of Native Dendrobium species in Thailand by PCRRFLP of the rDNA-ITS and Chloroplast DNA. Sci. Asia 40: 113-120.
              17. Srikulnath, K., Y. Uno, C. Nishida and Y. Matsuda. 2013. Karyotype evolution in monitor lizards: cross-species chromosome mapping of cDNA reveals highly conserved synteny and gene order in the Toxicofera clade. Chromosome Res. 21(8): 805-819.
              18. Chaiprasertsri, N., Y. Uno, S. Peyachoknagul, O. Prakhongcheep, S. Baicharoen, S. Charernsuk, C. Nishida, Y. Matsuda, A. Koga and K. Srikulnath. 2013. Highly species-specific centromeric repetitive DNA sequences in lizards: molecular cytogenetic characterization of a novel family of satellite DNA sequences isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota). J. Hered. 104(6): 798-806.
              19. Wannapinpong, S., K. Srikulnath, A. Thongpan, K. Choowongkomon and S. Peyachoknagul. 2013. Molecular cloning and characterization of the CHS gene family in turmeric (Curcuma longa Linn.). J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0232-8
              20. Islam, F.B., S. Ishishita, Y. Uno, M.B.R. Mollah, K. Srikulnath and Y. Matsuda. 2013. Male hybrid sterility in the mule duck is associated with meiotic arrest in primary spermatocytes. J. Poultry Sci. 50: 311–320.
              21. Srikulnath, K. 2013. The dynamics of chromosome evolution in reptiles. Thai J. Genet. S(1): 77-79.
              22. Thongtam Na Ayudhaya, P., C. indananda, S. Peyachoknagul and K. Srikulnath. 2013. Mitochondrial genome structure of saddleback anemonefish (Amphiprion Polymnus). Thai J. Genet. S(1): 343-346.
              23. Prakhongcheep, O., A. Swatdipong, C. indananda, S. Peyachoknagul and K. Srikulnath. 2013. Mitochondrial genome analysis of Siamese fighting fish Betta splendens. Thai J. Genet. S(1): 119-121.
              24. Prakhongcheep, O., N. Chaiprasertsri, S. Terada, Y. Hirai, K. Srikulnath, H. Hirai and A. Koga. 2013. Heterochromatin blocks constituting the entire short arms of acrocentric chromosomes of Azara’s Owl Monkey: formation processes inferred from chromosomal locations. DNA Res. doi:10.1093/dnares/dst023.
              25. Prakhongcheep, O., Y. Hirai, T. Hara, K. Srikulnath, H. Hirai and A. Koga. 2013. Two types of alpha satellite DNA in distinct chromosomal locations in Azara's Owl Monkey. DNA Res. doi:10.1093/dnares/dst004
              26. Srikulnath, K., A. Thongpan, S. Suputtitada and S. Apisitwanich. 2011. New haplotype of the complete mitochondrial genome of Crocodylus siamensis and its speciesspecific DNA markers: Distinguishing C. siamensis from C. porosus in Thailand. Mol. Bio. Rep. 39: 4709-4717
              27. Srikulnath, K., Y. Uno, K. Matsubara , A. Thongpan, S. Suputtitada, S. Apisitwanich, C. Nishida and Y. Matsuda. 2011. Chromosomal localization of 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae,Squamata). Genet. Mol. Biol. 34(4): 582-586.
              28. Unajak, S., P. Meesawat, K. Anyamaneeratch, D. Anuwareepong, K. Srikulnath and K. Choowongkomon. 2011. Identification of species (meat and blood samples) using nested-PCR analysis of mitochondrial DNA. Afr. J. Biotechnology 10(29): 5670-5676.
              29. Srikulnath K. 2010. FISH as a chromosome identification strategy to delineate karyotypic evolution in vertebrates. Thai J. Genet. 3(2): 120-136.
              30. Srikulnath K., K. Matsubara, Y. Uno, A. Thongpan, S. Suputtitada, C. Nishida, Y. Matsuda and S. Apisitwanich. 2010. Genetic Relationship of three butterfly lizard species (Leiolepis reveesii rubritaeniata, Leiolepis belliana belliana, Leiolepis boehmei, Agamidae Squamata) inferred from nuclear gene sequence analysis. Kasetsart J. (Nat. Sci.) 44: 424-435.
              31. Srikulnath K., C. Nishida, K. Matsubara, Y. Uno, A. Thongpan, S. Suputtitada, S. Apisitwanich and Y. Matsuda. 2009. Karyotypic evolution in squamate reptiles: comparative gene mapping revealed highly conserved linakage homology between the butterfly lizard (Leiolepis reveesii rubritaeniata, Agamidae, Lacertilia) and the Japanese four-strip rat snake (Elaphe quadrivergata, Culobridae, Serpentes). Chromosome Res. 17: 975-986.
              32. Srikulnath K., K. Matsubara, Y. Uno, A. Thongpan, S. Suputtitada, S. Apisitwanich, Y. Matsuda and C. Nishida. 2009. Karyological characterization of the butterfly lizard (Leiolepis reveesii rubritaeniata, Agamidae, Squamata) by molecular cytogenetic approach. Cytogenet. Genome Res. 125: 213-223.

              Proceedings in Conference

              1. Chaiprasertsri, N., A. Supikamolseni, S. Kamolnorranath, K. Kunya, S. peyachoknagul and K. Srikulnath. 2013. Molecular barcoding of varanid lizard in Thailand. In the 39th Congress on Science and Technology of Thailand (STT39). Bangkok, Thailand.
              2. Srikulnath, K. 2012. Snake genomics. In the conference "Snake Science" and Snake Venoms in Thailand". Bangkok, Thailand.
              3. Bonjongsat, N., P. Thongtam Na Ayudhaya, N. Ngaoburanawit, C. Indananda, S. peyachoknagul and K.Srikulnath. 2012. Sequence divergence of some anemonefishes in Thailand to verify species from each other. In the 50th Kasetsart University Annual
              Conference. Bangkok, Thailand.
              4. Srikulnath, K. 2011. Sex determination and sex chromosome in vertebrates. In 5th Wildlife and Zoo seminar. Bangkok, Thailand.
              5. Srikulnath, K.., A. Swatdipong, Y. Uno, K. Matsubara, S. Peyachoknagul and Y. Matsuda. 2011. Compartmentalization of retroelement (Rex1, Rex3 and Rex6) in Siamese
              fighting fish (Betta splendens, Anabantoidei, Perciformes) genome. In 17th National Genetic Conference. P 227-230. Chiangmai, Thailand.
              6. Supikamolseni, A., S. Suntrarachun, L. Chanhome, M. Sumontha, S. Peyachoknagul and K. Srikulnath. DNA barcoding of some venomous snakes in Thailand. In 17th National
              Genetic Conference. P 107-110. Chiangmai, Thailand.
              7. Charoenta, T., A. Swatdipong, V. Chailertrit, C. Indananda, P. Wonnapinij, K. Srikulnath and S. Peyachoknagul. Distinguish of three bubble nesting brooder fighting fishes by
              multiplex PCR. In 17th National Genetic Conference. P 231-234. Chiangmai, Thailand.
              8. Srikulnath, K., C. Nishida, K. Matsubara, Y. Uno, A. Thongpan, S. Suputtitada, Y. Matsuda and S. Apisitwanich. 2009. Comparative FISH chromosome mapping and
              nucleotide sequence analysis of 3 species butterfly lizards (Leiolepis reveesii rubritaeniata, Leiolepis belliana belliana, Leiolepis boehmei, Agamidae, Lacertilia) in
              Thailand, p. 76. In 35th Congress on Science and Technology of Thailand
              (STT35). Chonburi, Thailand.
              9. Srikulnath, K., A. Thongpan and S. Apisitwanich. 2007. Karyotypes of Siamese crocodile (Crocodylus siamensis) and saltwater crocodile (Crocodylus porosus) using Hetand
              G-banding. In 15th National Genetic Conference. p. 40-44. Songkhla, Thailand.

              Abstract in International Conference

              1. Srikulnath, K., K. Matsubara, Y. Uno, C. Nishida and Y. Matsuda. 2013. The dynamics of chromosome evolution in reptiles. 20th International Chromosome Conference. Bologna, Italy.
              2. Chaiprasertsri, N., Y. Uno, S. Peyachoknagul, O. Prakhongcheep, M. Shibusawa, S. Baicharoen, S. Charernsuk, C. Nishida, Y. Matsuda, A. Koga and K. Srikulnath. 2012. Molecular structure of repetitive element in the water monitor lizard (Varanus salvator macromaculatus, Platynota, Squamata). In 2012 Malasia-Thailand
              Graduate Forum in Life Science, Fodd Science, and Agriculture. Bangkok,
              Thailand.
              3. Srikulnath, K.., A. Swatdipong, Y. Uno, K. Matsubara, S. Peyachoknagul and Y. Matsuda. 2010. Localization of repetitive elements in Siamese fighting fish (Betta splendens, Anabantoidei, Perciformes) genome. In the abstract of 4th Asian Chromosome Colloquium. Beijing, China.
              4. Srikulnath, K., Y. Uno, K. Matsubara, A. Thongpan, S. Suputtitada, C. Nishida, Y. Matsuda and S. Apisitwanich. 2010. The complete mitochondrial genome of the three butterfly lizards (Leiolepis reveesii rubritaeniata, Leiolepis belliana belliana, Leiolepis boehmei, Agamidae, Squamata) and their genetic relationship in iguanian lizards, p.
              163. In The abstract of ISBDS2010 International Symposium on Biodiversity Sciences “Genome, Evolution and Environment”. Nagoya, Japan.
              5. Srikulnath, K., C. Nishida, K. Matsubara, Y. Uno, A. Thongpan, S. Suputtitada, S. Apisitwanich and Y. Matsuda. 2009. Chromosomal characterization of butterfly lizard, Leiolepis reveesii rubritaeniata). p. 46. In Chromosome science abstract The 3rd Asian Chromosome colloquium. Osaka, Japan.

              Abstract in Local Conference

              1. Sriwatananukulkit, O., S. Peyachoknagul and K. Srikulnath. Chromosomal karyotyping of striped catfish inferred from molecular cytogenetic approach. In the 39th Congress on Science and Technology of Thailand (STT39). Bangkok, Thailand.
              2. Supikamolseni, A., S. Suntrarachun, L. Chanhome, M. Sumontha, S. Peyachoknagul and K. Srikulnath. DNA marker for identification of some venomous snakes in Thailand. In the 39th Congress on Science and Technology of Thailand (STT39). Bangkok, Thailand.
              3. Suntronpong, A., S. Peyachoknagul and K. Srikulnath. 2013. Cytogenetic characterization of Barramundi (Lates calcarifer) using chromosome banding methods. In the 18th National Genetics Conference in Thailand. Bangkok, Thailand.
              4. Twilprawat, P., A. Suntronpong, S. Peyachoknagul and K. Srikulnath. 2013. Karyological characterization of swamp eel (Monopterus albus) inferred from molecular cytogenetic approach. In the 18th National Genetics Conference in Thailand. Bangkok, Thailand.
              5. Prakhongcheep, O., S. Lappharat, S. Baicharoen, B. Siriaroonrat, S. Kamolnorranath, R. Bunjongrat and K. Srikulnath. 2011. Karyological characterization of Eld’s deer and muntjacs in captive of Thailand. In 5th Wildlife and Zoo seminar. Bangkok,
              Thailand.
              6. Srikulnath, K., A. Swatdipong, C. Indananda and S. Peyachoknagul. 2010. Complete mitochondrial genome structure of wild Siamese fighting fish (Betta splendens, Anabantoidei, Perciformes) genome. In The abstract of 36th Congress on Science and Technology of Thailand (STT36). Bangkok, Thailand.

              Conference Organization

              2015 Secretary: The 5th Asian Chromosome Colloquium (New Horizon By Unifying of
              Chromosome Research) in Bangkok, Thailand during April 29 – May 1, 2015
              2015 Co-organizer: The 2nd UK-Japan chromosome structure workshop in Bangkok,
              Thailand during May 1, 2015

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