Associate Professor Dr. Pattana Srifah Huehne

  • Position:
    • Associate Professor/ Lecturer at Genetics Department
      Scientist Researcher at Chulabhorn Research Institute
  • Office number:
  • Lab number:
  • E-mail:,
  • Contacts:
    • Faculty of Science, Kasetsart University, Bangkok 10900 Thailand
      Tel. 66(0)2942-8716-7 and (0)2942-8390,
      Fax. 66(0)2579-5528

    • Laboratory of Biotechnology, Chulabhorn Research Institute, Vibhavadee Rd., Laksi, Bangkok 10210 Thailand
      Tel. 66(0)2574-0622 -33 ext 3817, Fax. 66(2)574-2027
  • Education:
  • B.Sc. (Plant Pathology) Kasetsart University, Bangkok, Thailand 1975-1978
    M.Sc. (Microbiology) Kasetsart University, Bangkok, Thailand1978-1981
    Ph D. (Virology) Australian National, Canberra, Australia 1988-1991

    Research interestsGrantsAdditional informationPublication
  • Plant Genetic Engineering in Orchids
  • Molecular Cloning and Proteomics in Orchids


    2011-2013Transformation of Scent Genes of Orange Jessamine and Kaffir Lime to
    Arabidopsis, Petunia and Orchid
    2011-2013Characterization of Bio-Molecules in Orchids Affecting Cancer Cell Activities
    2012-2013Evaluation of Genetics Diversity in Sesames using DNA Marker Specific to
    Genes Involving Fatty acid and Lignan Synthesis
    Kasetsart University Research and Development Institute (KURDI)
    2009-2011Use of antisense and RNAi technology to inhibit cymbidium mosaic virus
    2009-2010Use of antisense and RNAi technology to inhibit ethylene biosynthesis for
    long vase life of orchid flowers


      1. Lokkamlue N., Witcha Treesuwan, Pattana Srifah Huehne. 2012. Investigation of
      ethylene-sensitive properties from iron binding site of Vanda 1-
      aminocyclopropane-1-carboxylate oxidase by molecular modeling. Journal of
      Molecular Graphics and Modelling (submitted) 3/08/2012
      2. Lokkamlue, N., Huehne, P. S. 2012. Molecular Biology of Vanda Miss Joaquim 1-
      aminocyclopropane-1-carboxylate oxidase gene from the most ethylene-sensitive
      orchid flower. Maejo International Journal of Science and Technology.
      (submitted) 08/11/12
      3. Lokkamlue, N., Huehne ,P.S. 2012. Sequence Analysis of Ethylene Response
      Sensor Gene isolated from Vanda Miss Joaquim Flower, the Most Ethylenesensitive
      Orchid. Kasetsart Journal: Natural Science (submitted) KUJ: 55-10-12:
      4.Bhinija K, Srisomsap C, Chokchaichamnankit D, Svasti J, Mongkolsuk S, Huehne
      PS (2012) High resolution proteomic profiles generated from polysaccharide rich
      tissues of orchids. Planta (submitted) 15/11/12: pattana: huehne362722: PLAAD-12-00022
      1. Jantasuriyarat, C, Ritchuay, S,, Pattarawat ,P, Huehne, P.S., Kate-Ngam, S,. 2012.
      Development and transferability of EST-SSR and transferability of genomic SSR
      markers for genetic diversity assessment of Doritis. Biochemical Systematics and
      Ecology 45: 57-65.
      2. Huehne, P. S., Bhinija, K. 2012. Application of cryoprotectants to improve low
      temperature storage survival of orchid seeds. Scientia Horticulterae 135, 186-193.
      3. Wongkhamprai, B., Sangsavang, K. , Sriboonlert, A., Huehne, P. S. 2011.
      Efficiency of Agrobacterium transformation of pinene synthase gene isolated
      from kaffir lime in Arabidopsis thaliana. Thai Journal of Genetics 4(2): 85-93.
      4. Mohamad, Z.A., Chokchaichamnankit, D., Bhinija , K., Paricharttanakul, N. M.,
      Svasti, J., Huehne, P. S., Srisomsap, C. 2011. Proteomic analysis of Chinese kale
      (B. alboglabra) leaves during growth. Journal of Integrated Omics 1, 102-107
      5. Lertwiriyawong, B., Bhinija, K., Huehne, P.S., 2011. Sequence Analysis of the
      16SrRNA-rps12 Inverted Repeat Region in Chloroplast DNA of Dendrobium
      orchids . Kasetsart Journal - Natural Science 45, 461-72.
      6. Lertwiriyawong, B., Pinthong, K., Chanpaisaeng, J., Saksoong, P., Huehne, P.S.,
      2010. Analysis of the insecticidal crystal gene type 1 of Bacillus thuringiensis
      Thai isolates affecting lepidopterans. ScienceAsia 36, 271–279. doi:
      7. Phetkhong J. Srifah Huehne P. and Ngernsiri L. 2010. Cloning and sequencing
      analysis of alpha-amylase gene from black tiger prawn (Penaeus monodon). The
      Proceeding of 48th Kasetsart University Annual Conference 3-5 February 2010.
      p 336-343.
      8. Sangsavang, K. and Srifah Hunhne, P. 2009. Molecular cloning of germacrene-Dsynthase
      gene involving volatile fragrances of Kaffir lime. Proceedings of the
      16th National Genetics Conference. pp. 185-189. 25-27 March 2009, Thamasart
      University, Rungsit, Pathumthani.
      9. Soubsawwong, O and Srifah Hunhne, P. 2009. Molecular cloning of terpene
      synthase gene involving floral scent of orang Jessamine. Proceedings of the 16th
      National Genetics Conference. pp. 275-280. 25-27 March 2009, Thamasart
      University, Rungsit, Pathumthani.
      10. Sintuprapa, W., Theeragool, G., Yongmanitchai, W., Huehne, P.S., Matsushita,
      K., 2008 Molecular taxonomy of acetobacter syzygii SKU19 and
      characterization of its acetic acid adapted strains. Kasetsart Journal - Natural
      Science 42, 701-14.
      11. Suwanaketchanatit, C., Piluek, J., Peyachoknagul, S., Huehne, P.S., 2007. High
      efficiency of stable genetic transformation in Dendrobium orchid via
      microprojectile bombardment. Biologia Plantarum 51, 720-727.
      12. Huehne, P.S., P. Eurjirapongpun and C. Jampatong. 2006. Transformation of
      chitinase gene in Thai sweet corn inbred lines using particle bombardment and
      Agrobacterium. Agriculture Sci. J. 37(4): 375-384.
      13. Bhinija, K., S. Ruengverayut, S. Loprasert and P.S. Huehne. 2004. Development of a
      minimal medium to prolong the subculturing period of Vanda, dendrobium and
      Coelogyne orchids in vitro. Agriculture Sci. J. 35(3-4): 97-103.
      14. Arayaskul, N, P. Srifah and C. Piluek. 2003. Detection of potexvirus and tobamovirus
      causing diseases in orchids. Agriculture Sci. J. 33(4-5): 173-186.


      1.Petchthai, U., Peng, Du., Ding, Sho-wei, Huehne, P. S. 2012. MicroRNAs
      Expression of Ascocenda Orchid under Stress of Cymbidium Mosaic Virus
      Infection. Proceedings of 2nd World Congress on Virology, Track 11: Agriculture
      & Plant Virology on August 20-22, 2012, at Embassy Suites Las Vegas, USA.
      2.Wongkhamprai, B., Chatree, T., Roytrakul, S., Huehne, P.S. 2012. Identification of
      proteins from complex tissue of Cattleya flower by LC-MS. The 7th International
      symposium of the protein society of Thailand. 29-31 August 2012. Chulabhorn
      Research Institute Convention Center, Bangkok, Thailand.
      3.Bhinija, K., Chokchaichamnankit,D., Srisomsap ,C., Svasti, J., Huehne, P.S.(2012).
      The Identification of Mannose-Binding Lectins from Thai wild orchid,
      Bulbophyllum blepharistes Rchb.f. The 7th International symposium of the
      protein society of Thailand. 29-31 August 2012. Chulabhorn Research Institute
      Convention Center, Bangkok, Thailand.
      4. Huehne, P.S., S. Boonma, K. Phinij and J. Piluek. 2006. Odontoglossum ringspot
      virus-coat protein gene transformation into Dendrobium orchids. The
      proceeding of 44thKasetsart University Annual Conference. 30 January-2
      February, 2006. pp 109-116.
      5. Peyachoknagul, S., C. Mongkolsiiwatana, P.S. Huehne, N. Limsalukpet, S.
      Wannapinpong and E. Hitakomate. 2005. DNA typing of dendrobium samples
      by PCR-RFLP. The 14th National Genetic Conference, 11-13 March, 2005. Pp
      6. Lertwiriyawong, B., P. Saksoong, J. Chanpaiseang, and P.S. Huehne . 2005. Cloning
      of cryIE(a) gene effecting Spodoptera exigua in Thailand. The 14th National
      Genetic Conference, 11-13 March, 2005. Pp 426-431.
      7. Ketsuwan, U., S. Pasiri, J. Piluek, S. Peyachoknagul and P.S. Huehne. 2005. GFP
      gene transformation in Dendrobium orchid via particle bombardment. The 14th
      National Genetic Conference, 11-13 March, 2005. Pp 419-425.
      8. Ruengverayut S., K. Bhinija, S. Loprasert and P.S. Huehne. 2005. Effect of
      cytokinin on multiple shoots induction in Coelogyne, Dendrobium and Vanda
      orchids. In The Proceedings of 43 rd Kasetsart University Annual Conference
      (Subject:Plants), 1-4 February 2005, Bangkok

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